heart failure ; protein kinase C ; 493 ; Animals ; Diacylglycerol Kinase ; Drug Delivery Systems ; Gene Expression Regulation, Enzymologic ; Humans ; Hypertrophy ; Isoenzymes ; Mice ; Myocardial Infarction ; Myocytes, Cardiac ; Rats ; Signal Transduction ; Ventricular Remodeling
Description:
Diacylglycerol kinase (DGK) phosphorylates and converts diacylglycerol (DAG) to phosphatidic acid. DGK regulates cellular DAG levels and attenuates DAG signaling. The ten mammalian DGK isoforms have been identified to date. In cardiac myocytes, DGKα, ε, and ζ are expressed, and DGKζ is the predominant isoform. DGKζ inhibits protein kinase C activation and subsequent hypertrophic programs in response to endothelin-1 in neonatal rat cardiomyocytes. DGKζ blocks cardiac hypertrophy ...
Background and Objectives Oxygen permeability is important in platelet storage media. We compared a new polyolefin container with enhanced oxygen permeability (PO-80, Kawasumi, Tokyo, Japan) to a widely used alternative (PL2410, Baxter Healthcare, Illinois, USA). Materials and Methods In vitro characteristics of paired platelet concentrates (PCs, mean 4.2×1011/250 ml plasma/bag) stored in PO-80 or PL2410 were assessed through nine days of storage. In vivo recovery and survival ...
Culture-based bacterial detection systems for platelets: the effect of time prior to sampling and duration of incubation required for detection using aerobic culture
BACKGROUND: Bacterial contamination of platelet products (PLTs) occurs at low concentrations requiring a period of incubation for growth to minimize sampling error. Culture-based detection methods also need sufficient incubation time; together these periods may limit the useful life of PLTs. This study characterizes the impact of sampling and detection times using two commercially-available bacteria detection products. STUDY DESIGN AND METHODS: Apheresis PLTs inoculated with nine ...
BACKGROUND: Activation of the diacylglycerol (DAG)-protein kinase C (PKC) pathway has been implicated in the pathogenesis of a number of diabetic complications. Diacylglycerol kinase (DGK) converts DAG to phosphatidic acid and acts as an endogenous regulator of PKC activity. Akt/PKB is associated with a downstream insulin signaling, and PKCbeta attenuates insulin-stimulated Akt phosphorylation. METHODS AND RESULTS: We examined transgenic mice with cardiac-specific overexpression ...
EPR ; radical ; irradiation ; cross section ; Alanine ; Crystallography, X-Ray ; Electron Spin Resonance Spectroscopy ; Free Radicals ; Heavy Ions ; Linear Energy Transfer ; Radiation ; Sucrose
Description:
We investigated the sucrose radical-production cross section induced by heavy-ion irradiation. L-alanine was also used in order to compare radical yield and cross section. The stable free radicals after irradiation were analyzed by EPR (electron paramagnetic resonance). The radical yield obtained by the irradiated samples had a logarithmic correlation with the LET (linear energy transfer). Quantitative EPR analyses showed that radical productions for sucrose and L-alanine vary ...